Extended Data Fig. 4: GPX4 P159–OH inhibits CMA-mediated GPX4 degradation.
From: PSAT1 impairs ferroptosis and reduces immunotherapy efficacy via GPX4 hydroxylation
a,b, MDA-MB-231 cells transfected with the indicated shRNA were treated with IFNγ (25 ng ml−1) for 2 h and collected. c,d, MDA-MB-231 and BT549 cells expressing PHD3 shRNA with reconstituted expression of the indicated PHD3 proteins were collected for immunoblotting or were treated with or without IFNγ (25 ng ml−1) for 2 h. Immunoprecipitation and immunoblotting analyses were performed with the indicated antibodies. e, MDA-MB-231 and BT549 cells expressing PHD3 shRNA with reconstituted expression of the indicated PHD3 proteins were treated with CHX for the indicated time in the absence or presence of IFNγ and collected for immunoblotting. Data are the mean ± s.d., n = 6 independent experiments, two-tailed Student’s t test. f, MDA-MB-231 and BT549 cells expressing PHD3 shRNA with reconstituted expression of the indicated PHD3 proteins were treated with or without IFNγ (25 ng ml−1) for 9 h. Immunoblotting analyses were performed with the indicated antibodies. For all experiments, representative results from at least three independent experiments were shown.
