Extended Data Fig. 8: Manipulating PSAT1–GPX4 boosts antitumor immune response.
From: PSAT1 impairs ferroptosis and reduces immunotherapy efficacy via GPX4 hydroxylation
a–d, 4T-1 cells with expression of the indicated proteins were mammary pad-orthotopically injected into BALB/c female mice (n = 6 per group). These mice were given anti-mPD-1 antibody (100 µg/100 µl) or control hamster IgG (100 µg/100 µl) by i.p. injection starting on the seventh day after tumor inoculation and treated every 3 days a time for a total of five treatments. Intratumoral cells were collected for flow cytometry (FACS) analyses. Quantification for IFNγ+ CD8+ T cells, GzmB+ CD8+ T cells and perforin+ CD8+ T cells (a,b) and IFNγ+ CD4+ T cells, GzmB+ CD4+ T cells and perforin+ CD4+ T cells (c,d) were shown. Data are presented as the mean ± s.d., two-tailed Student’s t test.
