Extended Data Fig. 2: PSAT1 pS337 increases GPX4 stability.
From: PSAT1 impairs ferroptosis and reduces immunotherapy efficacy via GPX4 hydroxylation
a,d,e, Immunoprecipitation and immunoblotting were performed with the specified antibodies. All experiments were repeated three times independently with consistent results, and representative data are shown. a–c, The indicated cells were treated with IFNγ (25 ng ml−1) for the indicated periods and collected. Relative intensity of GPX4 to β-tubulin was analyzed (b). Data are the mean ± s.d., n = 6 independent experiments (two-tailed Student’s t test). The mRNA expression levels of GPX4 gene were measured using qPCR (c). Data are the mean ± s.d., n = 6 independent experiments (two-tailed Student’s t test). d,e, MDA-MB-231 and BT549 cells expressing PSAT1 shRNA with reconstituted expression of PSAT1 proteins were stably transfected with active CAMK2-CA. The indicated cells were treated with CHX for the indicated time and collected for immunoblotting. Relative intensity of GPX4 to β-tubulin was analyzed. Data are the mean ± s.d., n = 6 independent experiments, two-tailed Student’s t test.
