Extended Data Fig. 5: SDS-PAGE profile, color, and UV-vis profiles of purified and reconstituted proteins.
From: A heme-dependent enzyme forms the hydrazine in the antibiotic negamycin
a) SDS-PAGE (12%) analysis of NegJ. b) SDS-PAGE (18%) analysis of spFd6. SDS-PAGE analysis of NegJ and spFd6 was performed three times with similar results, and representative results are shown. c) Color differences of NegJ before and after reconstitution with hemin. d) Color differences of spFd6 before and after reconstitution of iron–sulfur cluster. e) α-band and β-band absorption peaks of purified NegJ in the reduced pyridine hemochromogen spectrum. f) The Soret peak of NegJ shifted from 414 nm to 417 nm when incubated with Gly and nitrite.
