Extended Data Fig. 10: Genome mining examples based on GNN of NegJ.
From: A heme-dependent enzyme forms the hydrazine in the antibiotic negamycin
The diagrams were created by EFI-GNDs online tool and edited with Inkscape 1.3.257. Proposed functions of genes neighboring to negJ homologs are highlighted and labeled. a) Partial Genome Neighborhood Diagram for cluster 1. The downstream oxygenase, hydroxylase, and methyltransferase genes indicate diverse structural modification of potential hydrazine based natural products. b) Partial Genome Neighborhood Diagram for cluster 3. The downstream genes encoding ATP-grasp domain containing proteins suggest the formation of amide bonds that connect hydrazine and other amino acids to produce novel N–N-bond containing compounds. c) Partial Genome Neighborhood Diagram for cluster 6. The upstream NRPS elements indicate the biosynthesis of a series of potential N–N-bond containing peptides.
