Extended Data Fig. 1: Nuclear ubiquitination permits Hippo–Yap signaling.
From: Nuclear ubiquitination permits Hippo–YAP signal for liver development and tumorigenesis
a, Evaluating the effect of global ubiquitination on TGF-β and Wnt/β-catenin signaling pathways. Data were presented as mean ± s.d. (n = 3 biological replicates), and P values were calculated by two-tailed unpaired Student’s t test. b, Screening of nuclear ubiquitin E3 ligases that regulate Hippo–Yap signaling. Data were presented as mean ± s.d. (n = 3 biological replicates), and p values were calculated by one-way ANOVA with Dunnett’s post hoc analysis, compared with shControl. ***P ˂ 0.0001. c, HEK293T cells transfected with HOP flash and UBR5 shRNAs were treated with 10 μM TRULI for 12 h and collected for luciferase measurement. Data were presented as mean ± s.d. (n = 3 biological replicates). The P values were calculated by one-way ANOVA with Dunnett’s post hoc analysis, compared with shControl. d, Evaluation of UBR5 knockdown efficiency in HepG2 cells. Images are representative of three independent experiments. e, HepG2 cells that stably integrated control and UBR5 shRNAs were stained for YAP (red) and DAPI (blue), and then subjected to confocal imaging. Images are representative of three independent experiments. Scale bar, 50 μm. f, YAP localization frequencies were quantified according to e. N > C, YAP is enriched in nucleus; N = C, YAP is evenly distributed in cytoplasm and nucleus; N < C, YAP is enriched in cytoplasm. Data were represented as mean ± s.d. (n = 3 biological replicates). The P values were calculated by one-way ANOVA with Dunnett’s post hoc analysis, compared with shControl. g, HepG2 cells that stably integrated with UBR5 shRNAs were transfected with HOP flash and collected for luciferase measurement. Data were represented as mean ± s.d. (n = 3 biological replicates). The P values were calculated by one-way ANOVA with Dunnett’s post hoc analysis, compared with control.
