Extended Data Fig. 6: Peroxisome-localized bergamottin production results in better host health and bergamottin titers compared to cytosolically-localized production.
From: Functional targeting of membrane transporters and enzymes to peroxisomes
(a) mTurquoise2 fluorescence of colonies used for bergamottin production in Fig. 5. As tCpPT1 was expressed as a fusion protein with mTurquoise2, the mTurquoise2 signal can be used as a proxy for protein expression. No significant differences in CpPT1 expression levels were observed with (SH3pep) versus without (dSH3pep) trafficking to the peroxisome. Two-way ANOVA was used. ns denotes not significant. Data are shown as the mean ± s.e.m. of three biological replicates. (b) A growth defect in yeast containing cytosolically-localized bergamottin biosynthesis (Cyt=pex5Δ) is observed when compared to peroxisome-compartmentalized (Per=WT) biosynthesis. OD600 values were taken at 72 h after dilution to an initial OD600 of 0.2. Two-way ANOVA was used. * denotes p-value < 0.05; *** p-value < 0.005. Data are shown as the mean ± s.e.m. of three biological replicates.
