Extended Data Fig. 1: The chauffeur strategy enables efficient trafficking of various MP cargoes to the peroxisome.
From: Functional targeting of membrane transporters and enzymes to peroxisomes
Venus-ePTS1 (YFP) was expressed to confirm the MP and the chauffeur localized to the peroxisome. (a) The yeast vacuolar MP, Vba2p, trafficked to peroxisomal membranes using the chauffeur strategy. In comparison, direct fusion of Vba2p to tPex22 did not result in efficient trafficking to the peroxisome; most of the Vba2p appeared to traffic to the vacuole. (b) CDT1p, a heterologous multispanning cellodextrin transporter from Neurospora crassa, trafficked to the peroxisome using the chauffeur strategy. The tPex22 direct fusion seemed to redirect CDT1p to the peroxisomal membrane; however, the total amount of CFP-fused MP successfully targeted to the peroxisome was much lower than achieved via the chauffeur strategy. Collectively, the results of trafficking Yea4p (Fig. 1b), Vba2p, and CDT1p suggest that the chauffeur strategy is a more robust method to redirect native and heterologous membrane proteins to the peroxisome. All scale bars represent 5 µm.
