Extended Data Fig. 9: Characterization of additional mutants of Lis1 at the PhiL-Lis1 interface.
From: Nde1 promotes Lis1 binding to full-length autoinhibited human dynein 1
a, MP profiles illustrate the interaction of Nde1 (N) with Lis1 mutants. Nde1 interacts with one (NL) or two (NL2) Lis1 dimers. b, MP of the binding of Lis1 mutants to open dynein. Dynein and Lis1 were incubated for 2 minutes at 1:2 ratio (D: dynein only, DL: one dynein and one Lis1). c. MP shows the binding of Lis1 mutants to dynein with or without Nde1. Dynein, Lis1 and Nde1 were incubated for 2 minutes at 1:2:2 ratio (DL2N: one dynein, two Lis1s, and one Nde1). d, Representative kymographs show the motility of WT DDR complexes with or without Nde1 and Lis1. e, Run frequency of WT DDR with or without Nde1 and Lis1 (mean ± s.d.; n = 30 MTs for each condition; statistics from two independent experiments). Results were normalized to the -Lis1, -Nde1 condition. P values are calculated from a two-tailed t-test.
