Extended Data Fig. 3: Quantification of Phi-dynein percentage in the presence or absence of Lis1 and Nde1.
From: Nde1 promotes Lis1 binding to full-length autoinhibited human dynein 1
Dynein (D) was incubated with Lis1 (L), Nde1 (N), or both proteins for 90 minutes at the indicated molar ratios of protein dimers. The percentage of Phi-dynein was quantified under each condition, D + L (a), D + N (b), D + L + N (c). Colors represent independent biological replicates. n = 6 biological replicates for D:L = 1:1 and D:L = 1:2; n = 3 biological replicates for D:N = 1:1 and n = 4 biological replicates for D:N = 1:2; n = 4 biological replicates for D:L:N = 1:1:1 and D:L:N = 1:2:2. The control (Ctrl) corresponds to the Phi-dynein percentage measured in the dynein-alone condition.
