Extended Data Fig. 5: Pharmacologically or genetically inhibition of ALDH2 on basal excitatory or inhibitory transmission.
From: ALDH2-mediated GABA biosynthesis regulates hippocampal LTP and learning adaptability
a, eEPSCs in pyramidal neurons were evoked at holding potential of -70 mV by the paired stimulation (Ctrl: n = 9 neurons from 3 mice; Cyanamide: n = 9 neurons from 3 mice; shALDH2: n = 8 neurons from 3 mice). b, Input-output curves measured of eEPSCs in hippocampal SC-CA1 synapses (Ctrl: n = 7 neurons from 3 mice; Cyanamide: n = 6 neurons from 3 mice; shALDH2: n = 6 neurons from 3 mice). c, Trace records of the sEPSC under different conditions. d, f, The quantitative frequency (d) or amplitude (f) of sEPSCs from CA1 PNs. e, g, Cumulative probability plot of sEPSCs frequency (e) or sEPSCs amplitude (g) (Ctrl: n = 18 neurons from 3 mice; Cyanamide: n = 20 neurons from 3 mice; shALDH2: n = 18 neurons from 3 mice). Statistical significance indicated as ‘ns’ for Ctrl vs. Cyanamide, and ‘NS’ for the comparison between Ctrl and shALDH2. h, Trace records of the sIPSC under different conditions. i, k, The quantitative frequency (i) or amplitude (k) of sIPSCs from CA1 PNs. j, l, Cumulative probability plot of sIPSCs frequency (j) or sIPSCs amplitude (l) (Ctrl: n = 20 neurons from 3 mice; Cyanamide: n = 17 neurons from 3 mice; shALDH2: n = 18 neurons from 3 mice). Statistical significance indicated as ‘*’ or ‘ns’ for Ctrl vs. Cyanamide, and ‘#’ or ‘NS’ for Ctrl vs. shALDH2. Data are represented as mean ± SEM. Statistics were calculated by one-way ANOVA with Tukey’s multiple comparison post hoc test (d, f, i, k) or Kolmogorov-Smirnov test (e, g, j, l). **P < 0.01, ****P < 0.0001. ns, not significant.
