Extended Data Fig. 6: Role of ALDH2-mediated increase in iGABA in modulating mIPSC of PNs.
From: ALDH2-mediated GABA biosynthesis regulates hippocampal LTP and learning adaptability
a, The quantitative frequency of GABA mIPSCs from CA1 PNs. b, c, Cumulative probability plot of mIPSCs frequency from CA1 PNs in Vglut2-Cre mice with intra-CA1 injection of AAV-DIO-scramble (shScr) (b) or AAV-DIO-shALDH2 (shALDH2) (c) (shScr: n = 24, 25 and 23; shALDH2: n = 23, 25 and 22). Statistical significance indicated as ‘*’ for Ctrl vs. GABA intra-pipette delivery, and ‘#’ for Ctrl vs. LTP. Each dot represents one neuron and all neurons measured from 4 mice. d, The quantitative amplitude of GABA mIPSCs from CA1 PNs in the presence of NNC-711 (Ctrl: n = 18 neurons from 3 mice; n = 17 neurons from 4 mice for other groups). e, Cumulative probability plot of mIPSCs amplitude from CA1 PNs in the presence of NNC-711. Statistical significance indicated in blue for the comparison between the Ctrl and GABA intra-pipette comparison, and red for the comparison between Ctrl and LTP comparisons. f, The quantitative AP threshold with different treatment (shScr: n = 17, 20 and 20; shALDH2: n = 21, 18 and 21). Each dot represents one neuron and all neurons measured from 3 mice. g, h, The trace (g) and spike number (h) of the CA1 PNs with different treatment in the presence of 20 µM Bicuculline (Ctrl, n = 20 neurons; GABA intra-pipette, n = 21 neurons; LTP, n = 21 neurons). All neurons measured from 3 mice. Data are represented as mean ± SEM. Statistics were calculated by two-way (a, f), Kolmogorov-Smirnov test (b, c, e), one-way ANOVA (d, h) with Tukey’s multiple comparison post hoc test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. ns, not significant.
