Extended Data Fig. 2: Single-cell metabolomics analysis confirms the structure of iGABA and its related metabolites in single hippocampal PNs.
From: ALDH2-mediated GABA biosynthesis regulates hippocampal LTP and learning adaptability
a-h, MS/MS spectra for GABA (a), ABAL (b), Glutamine (c), Glutamate (d), Proline (e), Aspartate (f), Arginine (g) and NAA (h) from single hippocampal PNs (top) and standards (bottom). Higher-energy collisional dissociation (HCD) at 35% energy. i, GABA calibration curve constructed by plotting MS intensity/Total Ion Chromatography (TIC) against the concentration of the GABA standard. The area shows the range of intracellular GABA concentrations in single CA1 PNs (cyan) or interneurons (red). j, The concentrations of GABA intensity in CA1 PNs or interneurons (PNs, n = 14 neurons; Interneurons, n = 17 neurons). Neurons pooled from 3 mice. Data are represented as mean ± SEM. Statistics were calculated by unpaired two-tailed t test (j). ****P < 0.0001.
