Extended Data Fig. 1: Impact of tau fragments on the aggregation kinetics of Aβ.
From: Interactions with tau’s microtubule-binding repeats modulate amyloid-β aggregation and toxicity
a, Analysis of the aggregation kinetics of Aβ40 incubated with different concentrations of R1, R4, PHF6*, and PHF6. The ThT intensity of tau fragments is presented with triangles. b, Values of tlag and t1/2. These values were calculated by fitting the ThT emission with a sigmoidal equation45. Conditions: [tau fragment] = 10, 50, and 100 μM; [Aβ40] = 10 μM; [ThT] = 5 μM; 20 mM HEPES, pH 7.4, 150 mM NaCl; 37 °C; 250 rpm; λex = 440 nm; λem = 490 nm. All values in the ThT-sigmoidal graphs are indicated as mean ± s.e.m. for n = 7 examined over three independent experiments. The error values of tlag and t1/2 represent the fitting error.
