Extended Data Fig. 3: Cytotoxicity of Aβ incubated with tau fragments.
From: Interactions with tau’s microtubule-binding repeats modulate amyloid-β aggregation and toxicity
Cell survival (%) was calculated in comparison to that with an equivalent amount of the buffered solution. Conditions: [Aβ40] = 10 μM; [tau fragment] = 10, 50, and 100 μM; 20 mM HEPES, pH 7.4, 150 mM NaCl; 37 °C. All values are indicated as mean ± s.e.m. for n = 6 examined over three independent experiments. The P values for Aβ40 with PHF6* or PHF6 are summarized: for PHF6* (10 equiv, P = 0.0038); for PHF6 (10 equiv, P = 0.0363). The P values for tau fragments with Aβ40 are obtained: for R1 (1 equiv, P = 2.9 × 10−11; 5 equiv, P = 4.7 × 10−14; 10 equiv, P = 1.2 × 10−11); for R4 (1 equiv, P = 4.1 × 10−12; 5 equiv, P = 3.8 × 10−9; 10 equiv, P = 9.3 × 10−10); for PHF6* (1 equiv, P = 2.2 × 10−13; 5 equiv, P = 2.4 × 10−11; 10 equiv, P = 7.4 × 10−10); for PHF6 (1 equiv, P = 5.1 × 10−10; 5 equiv, P = 1.0 × 10−10; 10 equiv, P = 1.7 × 10−11). *P < 0.05, **P < 0.01, or ****P < 0.0001 by a two-sided unpaired Student’s t-test.
