Extended Data Fig. 3: Cryo-EM maps and structures of the active site of T. thermophilus transcription initiation complexes containing Np4-capped di- and trinucleotide RNAs.
From: Molecular basis for noncanonical transcription initiation from Np4A alarmones
The figures focus on the transcription products (initiating Np4As in magenta and other nucleotides in pink) and tDNA (colored as in Fig. 2) shown with schematics of the RNA-template interactions (top), matching maps in mesh representation (middle) and zoomed-in top views of the maps and models of the cap moieties (bottom or right). a, ATP-C(-1dC) complex. b, Gp4A-C(-1dC) complex. c, Gp4A-C(-1dA) complex. d, Ap4A-C(-1dC) complex. e, Ap4A-C(-1dC) complex. f, Up4A-C(-1dA) complex. g, Gp4A-C-U(-1dC) complex. To show map quality with reduced background, the map contour level was set higher in the zoomed-in images (bottom) than in the overall views (top) by ~10 % (b, f) or ~20 % (d, e).
