Supplementary Fig. 2: Antibody responses elicited by hemagglutinin RBD–np and commercial vaccines to influenza virus.
From: Mosaic nanoparticle display of diverse influenza virus hemagglutinins elicits broad B cell responses
Serum HAI and neutralization antibody titers to CA09 (a) and BR07 (b). BALB/c mice were immunized with NC99 RBD–np (valence 1) or various preparations of various RBD–np either as admixtures (admix) or as co-assembled particles (Mosaic). Represented data were generated using sera collected 2 weeks after the second immunization with adjuvant (2 µg of RBD–np per immunization). Shown results were obtained from a representative experiment (n = 5). Each dot indicates individual mouse with mean of each group indicated as lines. Animal experiments were independently performed two times with similar results. Statistical analyses are done with one-way ANOVA with Tukey’s multiple comparisons post-hoc test. Non-statistical significance between groups is not shown for clarity. (c) Microneutralization (MN) titers against WS33, KW86, and CC03 viruses of pooled immune sera. Mice (n = 5) were immunized with 2 µg of mosaic RBD–np (8-valent), or 1.5 µg of commercial TIV (from indicated season) formulated with SAS and delivered at weeks 0 and 4. Immune sera were collected at week 6, RDE-treated, heat-inactivated, and pooled for microneutralization assays. Animal experiment was performed once.
