Supplementary Fig. 3: Hemagglutinin-binding and H1N1 neutralization of monoclonal antibodies.
From: Mosaic nanoparticle display of diverse influenza virus hemagglutinins elicits broad B cell responses
(a) Hemagglutinin-binding of monoclonal antibodies measured by biolayer interferometry. Recombinant hemagglutinin trimer proteins were immobilized on HIS1K biosensors through hexa-histidine tag and used to assess binding of monoclonal antibodies. All assays were performed in the Octet HTX instrument (fortéBio) at 30ºC with agitation at 1,000 rpm. PBS with 1% BSA was used as assay buffer for all steps. Plotted data indicate the response value during the mAb association step at 300 s. Control monoclonal antibodies 3u-u, 2D1 and CH65 were shown as gray bars. Experiments were performed two times with similar results. (b) Neutralization capacity of monoclonal antibodies. Pseudotype neutralization assays were used to assess neutralization potency of isolated monoclonal antibodies. Homologous NC99 and heterologous BJ95 strains were selected for screening. Experiments were performed two times with similar results.
