Extended Data Fig. 8: Pulmonary FSC-derived IL-33 preserves metabolic and functional fitness of inflationary memory CD8⁺ T cells.

(a to d, Ccl19-Cre-EYFP/iDTR mice were immunized i.v. with Ad-LacZ/FlexON and DT or PBS was given intranasally on day 3 and 5. Single cell RNA-seq analysis of bgal₉₆ tetramer⁺ CD8⁺ T cells isolated from lungs on day 21. (a) Network plots displaying most significantly enriched gene ontologies based on transcriptional differences in bgal₉₆ tetramer⁺ CD8⁺ T cells from DT vs PBS treated Ccl19-Cre/iDTR EYFP mice and with enriched genes annotated. (b) Violin plots show expression of Atp5b, Cox5a, Cox8a, Tomm20. (c) Representative FACS histograms of Ki67 staining on pulmonary bgal₉₆ tetramer⁺ CD8⁺ T cells (left) and frequency of Ki67⁺ bgal₉₆-specific CD8⁺ T cells (right). (d) Representative FACS dot-plot of live-dead stain negative pulmonary bgal₉₆ tetramer⁺ CD8⁺ T cells (left) and frequency of Aqua-negative bgal₉₆-specific CD8⁺ T cells (right). e, Quantitative real-time PCR analysis of Sdha, Uqcrc2 and Cox4i2 expression in bgal₉₆-specifc CD8⁺ T cells sorted from the lung of Ccl19-Cre⁺ Il33^fl/fl and Ccl19-Cre⁺ Il33^+/+ mice, day 21 after Ad-LacZ/FlexON immunization. Dots represent individual mice. Bar graphs indicate mean ±s.e.m. (c-e) Pooled data from 2 independent experiments with n = 8 mice per group [(c)]; n = 7 mice [(d)]; and n = 4 mice [(e)]. Statistical analysis was conducted using unpaired two-tailored Student’s t test with *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Exact P values are provided in the Source Data.

Source data