Extended Data Fig. 9: Kinetics and tissue distribution of inflationary memory CD8+ T cells under conditions of Ccl19-Cre+ lung FSC depletion and Il33-ablation in Ccl19-Cre+ cells.
Ccl19-Cre-EYFP/iDTR mice and Ccl19-Cre Il33fl/fl mice (Il33fl/fl) were immunized i.v. with Ad-LacZ/FlexON and CD8+ T cells were analyzed on day 21. Ccl19-Cre-EYFP/iDTR mice were treated i.n. with DT or PBS on day 3 and 5 after immunization. a, Number of bgal96 tetramer+ CD8+ T cells in lung and spleen. b, Frequency of bgal497 tetramer+ CD8+ T cells in blood, lung and spleen. c, Frequency of KLRG1+/CX3CR1+ bgal497 tetramer+ CD8+ T cells in the lungs. d, IFN-g- and TNF-producing pulmonary bgal497-specific CD8+ T cells. Dots represent individual mice. Bar graphs indicate mean ±s.e.m. Pooled data from 2 independent experiments with n = 9 (DT intranasal), n = 9 (Ccl19-Cre Il33fl/fl) and n = 19 (Ccl19-EYFP/iDTR PBS i.n. and Ccl19-Cre Il33+/+) mice [(a)]; n = 9 (DT intranasal), n = 8 (Ccl19-Cre Il33fl/fl) and n = 19 (Ccl19-EYFP/iDTR PBS i.n. and Ccl19-Cre Il33+/+) mice [(b)]; n = 6 (DT intranasal), n = 9 (Ccl19-Cre Il33fl/fl) and n = 16 (Ccl19-EYFP/iDTR PBS i.n. and Ccl19-Cre Il33+/+) mice [(c)]; n = 7 (DT intranasal), n = 8 IFNg, n = 6 TNF (Ccl19-Cre Il33fl/fl) and n = 18 (Ccl19-EYFP/iDTR PBS i.n. and Ccl19-Cre Il33+/+) mice [(d)]. Statistical analysis was conducted using one-way ANOVA with Tukey’s multiple comparison test with *P < 0.05; **P < 0.01; ***P < 0.001, ****P < 0.0001. Exact P values are provided in the Source Data.
