Extended Data Fig. 1: Adenoviral vectors ChAdOx1 and HuAd5 transduce human fibroblasts.
a, Preparation of sliced tissue cultures from human palatine tonsils following infection with adenoviral vectors. b, Flow cytometry-based gating strategy for PBMCs analysis. c and d, Frequency of CD3+ T cells, CD14+ HLA-DR+ monocytes or CD11c+ dendritic cells (DCs) within GFP+ cells following infection with (c) ChAdOx1-GFP or (d) HuAd5-GFP. e, Flow cytometry-based gating strategy for cultured tonsillar tissue stromal cell analysis. f and g, Frequencies of PDPN+ and CD31+ cells within GFP+ cultured tonsillar tissue stromal cells (TSC) following infection with (f) ChAdOx1-GFP or (g) HuAd5-GFP. h to m, Infection of cultured (h to j) skin- or (k to m) lung-derived stromal cells with ChAdOx1-GFP or HuAd5-GFP with representative FACS plots. (i and l) Frequency of GFP+ cells after infection with indicated adenoviral vectors at different multiplicity of infection (MOI). (j and m) Frequency of PDPN+ or CD31+ cells within GFP+ cells after infection with indicated adenoviral vectors. Pooled data from n = 3 PBMC samples [(c) and (d)] and n = 3 TSC samples [(f) and (g)]. Data from one experiment with single skin [(h-j)] or lung tissue donor [(k-m)]. Statistical analysis was performed using one-way ANOVA with Tukey’s multiple comparison test [(c) and (d)] and unpaired two-tailored Student`s t test [(f) and (g)], with *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Exact P values are provided in the Source Data.
