Extended Data Fig. 2: Induction of inflationary memory CD8+ T cells following Ad5-LacZ immunization.
a to c, B6 mice were immunized i.v. with Ad-LacZ. (a) Kinetics of the frequency of bgal96 or bgal497 tetramer+ CD8+ T cells in blood. Frequency of bgal96 or bgal497 tetramer+ CD8+ T cells in indicated organs on (b) day 21 or (c) day 50. d, Flow cytometry-based gating strategy for tetramer+ CD8+ T cell analysis. e, Ubi-Cre ERT2 mice were immunized i.v. with Ad-LacZ/FlexON. Frequency of bgal96 or bgal497 tetramer+ CD8+ T cells in blood after application of Tamoxifen. (f to g) LysM-Cre mice were immunized i.v. with Ad-LacZ/FlexON. f, Kinetics of bgal96 tetramer+ CD8+ T cell response with representative FACS plots. g, Kinetics of non-inflationary bgal497 tetramer+ CD8+ T cell response with representative FACS plots. Cre-negative mice were used as controls (Ctrl). Pooled data from 2 independent experiments with n = 4-8 mice [(a)]; n = 4 mice per group [(b) and (c)], representative of two experiments with n = 2 per group [(e)], n = 8 (LysM-Cre+) and n = 8 (Ctrl) [(f) and (g)]. Values indicate mean±s.e.m. for each time point. Statistical analysis was performed using two-way analysis of variance (ANOVA) with Bonferroni multiple comparison test [(f) to (g)] with *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Exact P values are provided in the Source Data.
