Extended Data Fig. 3: Ablation of Nrp1 impairs anti-helminth immunity in Id2cre-ERT2Nrp1f/f mice.
From: Neuropilin-1 mediates lung tissue-specific control of ILC2 function in type 2 immunity
a-i Id2cre-ERT2Nrp1f/+ and Id2cre-ERT2Nrp1f/f mice were injected intraperitoneally with tamoxifen (2 mg/day per mouse) for 5 days to knock out Nrp1 expression in the ILC lineage. 3 days later, these mice were infected with 500 L3 N brasiliensis. Lung tissues were collected and analyzed 5 days after worm infection. (a) Intracellular staining of IL-5 and IL-13 in lung ILC2s. (b, c) Frequency and absolute account of IL-5+ ILC2s in lung. (d, e) Frequency and absolute account of IL-13+ ILC2s in lung. (f) Total number of lung ILC2s. (n = 7 for black group and n = 8 for red group in b-f) (g) Flow cytometric analysis of eosinophils (CD45+Ly6G−CD11b+SiglecF+) in lung. Cells were gated on CD45+Ly6G− live single cells. (h) Absolute count of lung eosinophils. (i) Worm burden in the small intestine collected on day 7 post infection. (n = 8 for black group and n = 9 for red group in h-i) Each symbol represents an individual mouse (b-f, h, and i). Data are representative of 3 independent experiments and are presented as mean ± SEM. Two-tailed unpaired Student’s t test.
