Extended Data Fig. 5: Loss of Nrp1 reduces ILC2 function and anti-helminth immunity in R5/ + Nrp1f/f mice.
From: Neuropilin-1 mediates lung tissue-specific control of ILC2 function in type 2 immunity
a-i R5/ + and R5/ + Nrp1f/f mice were infected with 500 L3 N brasiliensis subcutaneously. Lung tissues were collected and analyzed 5 days after worm infection. (a) Intracellular staining of IL-5 and IL-13 in lung ILC2s. (b, c) Frequency and absolute account of IL-5+ ILC2s in lung (n = 5/group). (d, e) Frequency and absolute account of IL-13+ ILC2s in lung (n = 5/group). (f) Total number of lung ILC2s (n = 5/group). (g) Flow cytometric analysis of eosinophils in lung. Cells were gated on CD45+ CD11b+Ly6G− live single cells. (h) Absolute count of lung eosinophils (n = 5/group). (i) Worm burden in the small intestine collected on day 7 post infection (n = 5/group). Each symbol represents an individual mouse (b-f, h, and i). Data are representative of 3 independent experiments and are presented as mean ± SEM. Two-tailed unpaired Student’s t test.
