Extended Data Fig. 6: Nrp1 deficiency leads to reduced ILC2 function during pulmonary fibrosis.
From: Neuropilin-1 mediates lung tissue-specific control of ILC2 function in type 2 immunity
(a) R5/ + and R5/ + Nrp1f/f mice were challenged with bleomycin to induce pulmonary fibrosis. Mice were sacrificed on day 21 and lung tissues were collected. Flow cytometric analysis of IL-5+ lymphocytes. Cells were gated on live CD45+ lymphocytes. b-e ILC2s isolated from lung and large intestine of R5/ + or R5/ + Nrp1f/f mice were transferred intravenously into Rag2–/–Il2rg–/– mice, respectively. After 7 days, the recipient mice were given bleomycin intranasally. Lung tissues were collected and analyzed on day 21 of fibrosis. (b, c) Frequency and absolute number of lung ILC2s (n = 4/group). (d, e) Frequency and absolute number of IL-5+ ILC2s in lung (n = 4/group). Each symbol represents an individual mouse (b-e). Data are representative of 2-3 independent experiments and are presented as mean ± SEM. Two-tailed unpaired Student’s t test.
