Extended Data Fig. 8: Treatment with EG00229 intranasally alleviates pulmonary fibrosis.
From: Neuropilin-1 mediates lung tissue-specific control of ILC2 function in type 2 immunity
a-i Bleomycin-challenged mice were treated with EG00229 (2.5 mg/kg body weight in 20μl PBS) or DMSO control intranasally three times weekly for three weeks. (a) The survival rate of mice treated with EG00229 or DMSO control was observed for a period of 21 days (n = 16/group). (b, c) H&E staining and Masson’s trichrome staining of lung tissues. (d) mRNA expression of the fibrotic genes in lung (n = 3, 6, 6 for PBS, DMSO, and EG00229 group). (e, f) Frequency and absolute count of IL-13+ ILC2s (n = 6/group). (g, h) Frequency and absolute count of IL-5+ ILC2s (n = 6/group). (i) Total number of α-SMA+ myofibroblast (n = 6/group). Each symbol represents an individual mouse (d-i). Data are representative of at least 3 independent experiments and are presented as mean ± SEM. (a) Log-rank test. (d-i) Two-tailed unpaired Student’s t test.
