Extended Data Fig. 1: Nrp1 is a tissue-specific marker of lung ILC2s.
From: Neuropilin-1 mediates lung tissue-specific control of ILC2 function in type 2 immunity
(a) Box plot compared the expression of NRP1 in human ILC2s from lung (n = 6) and spleen (n = 5). The bulk RNA-seq data were obtained from Gene Expression Omnibus (GEO) under accession code GSE126107. The center line, lower whisker, and upper whisker stand for median, minima, and maxima, respectively. Bounds of box are 25-75th percentile. (b) Gating strategy of ILC2s. ILC2s were gated on live and single cells with lin−(CD3−B220−CD11b−CD11c−CD5−) CD45+CD127+GATA3+. (c) The compensation of 15-color (including 19 markers) flow cytometric panel was shown by 14×14 view. Cells were gated on single lymphocytes. (d) ILC2s sorted from large intestine were cultured with TGFβ1 at 1 ng/ml for 48 h. mRNA expression level of Nrp1 was analyzed by quantitative RT-PCR (n = 3/group). Each symbol represents an individual mouse (d). Data are representative of 3 independent experiments. Two-tailed unpaired Student’s t test.
