Extended Data Fig. 2: Loss of Nrp1 does not alter lung ILC2 homeostasis in Id2cre-ERT2Nrp1f/f mice under steady state.
From: Neuropilin-1 mediates lung tissue-specific control of ILC2 function in type 2 immunity
a-m Id2cre-ERT2Nrp1f/+ and Id2cre-ERT2Nrp1f/f mice were injected intraperitoneally with tamoxifen (2 mg/day per mouse) for 5 days to knock out Nrp1 expression in the ILC lineage. (a-c) The expression level of Nrp1 on Treg, DCs, and neutrophils from lung were analyzed by flow cytometry. (a) The top row was gated on CD45+ live cells. (b) The top row was gated on CD45+CD11b+F4/80−Ly6G− live cells. (c) The top row was gated on CD45+ live cells. (d-f) Absolute cell counts of lung Treg, DCs, and neutrophils (n = 3/group). (g) The expression level of Nrp1 on lung ILC2s were analyzed by flow cytometry. Cells were gated on lin−CD45+CD127+ lymphocytes. (h, i) H&E staining and Masson’s trichrome staining of lung tissue. (j) Absolute cell counts of lung ILC2s (n = 11/group). (k) Percentage of IL-5+ ILC2s and IL-13+ ILC2s. Cells were gated on total ILC2s. (l, m) Frequency and total number of IL-5+ ILC2s (n = 10/group) and IL-13+ ILC2s (n = 7/group) Each symbol represents an individual mouse (d-f, j, l, and m). Data are representative of 3-4 independent experiments and are presented as mean ± SEM.
