Extended Data Fig. 1: Sex differences in IFN-γ production in response to IL-12/18.
From: The X-linked epigenetic regulator UTX controls NK cell-intrinsic sex differences
a) Representative dot plots showing gating strategy to identify CD3− TCRβ- NK1.1+ mouse NK cells. b) Representative contour plots, (c) percentage IFN-γ+, and (d) normalized IFN-γ MFI of female and male WT NK cells with cultured no treatment (NT) or IL-12 (20 ng/mL) and IL-18 (10 ng/mL) for 4 hours (n = 14 per group). e) Representative contour plots of CD3−CD56+ female (n = 6) and male (n = 7) human NK cells cultured and stimulated with 10 ng/mL of IL-12 for 16 hours in the presence of K562 cells. f) Representative dot plots of splenic NK cells (CD3−TCRβ- NK1.1+) in gonadectomized female and male mice. g) Representative contour plots of total splenic NK cells isolated from gonadectomized female and male mice and cultured with no treatment (NT) or IL-15 (50 ng/mL) and IL-12 (20 ng/mL) for 4 hours. h) Representative contour plots and i) percentage CD27−CD11b− (DN), CD27+CD11b− (CD27 SP), CD27+CD11b+ (DP), and CD27−CD11b+ (CD11b SP) of total splenic NK cells from female and male mice (n = 7 per group). j) Representative contour plots and k) percentage CD27−CD11b− (DN), CD27+CD11b− (CD27 SP), CD27+CD11b+ (DP), and CD27−CD11b+ (CD11b SP) of total splenic NK cells from gonadectomized mice (n = 6 per group). Data are representative of 2-3 independent experiments. Samples were compared using unpaired two-tailed Student’s t test and data points are presented as individual mice with the mean ± SEM (N.S., Not Significant; **, p < 0.01; ****, p < 0.0001). Specific p-values are as follows: c:[NT = 0.987; IL-12+IL-18 = 0.00125]; d:[NT = 0.4144; IL-12+IL-18 < 0.0001]; i:[DN > 0.999; CD27 SP = 0.9514; DP = 0.8995; CD11b SP = 0.6517]; k:[DN = 0.0951; CD27 SP = 0.225; DP = 0.161; CD11b SP = 0.789].
