Extended Data Fig. 2: UTX expression in Four Core Genotypes mice and maturation in UTX mouse models.
From: The X-linked epigenetic regulator UTX controls NK cell-intrinsic sex differences
a) Relative expression of Kdm6a (encodes the protein UTX) by RT-qPCR in Four Core Genotypes mice, in which male or female gonads present are independent of XX or XY chromosome composition, normalized to female WT expression (XX with ovaries) (XX ovaries: n = 4; XY ovaries: n = 5; XX testes: n = 3; XY testes: n = 5). b) Relative UTX MFI in splenic NK cells isolated from female WT (n = 19), male WT (n = 19), female UTXHet (n = 7), and female UTXNKD (n = 4) mice. c) CD11b and CD27 expression within NK cells isolated from female WT (n = 13), male WT (n = 7), and female UTXHet (n = 8) mice. d) Representative western blot showing protein expression of UTX in NK cells isolated from female WT and UTXNKD mice compared to β-actin loading control. Data are representative of 2-3 independent experiments. Samples were compared using a) unpaired two-tailed Student’s t test, b,c) one-way ANOVA with Tukey’s correction for multiple comparisons. Data points are presented as individual mice with the mean ± SEM (N.S., Not Significant; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001). Specific p-values are as follows: a:[Ovaries – XX vs. XY < 0.0001; Testes – XX vs. XY = 0.0016]; b:[F WT vs. M WT = 0.003; F UTXNKD vs. F UTXHet and F WT vs. F UTXNKD < 0.0001; M WT vs. F UTXHet = 0.7435; F UTXHet vs. F WT = 0.0004]; c > 0.999.
