Extended Data Fig. 5: RSTR T_SCM cells proliferate similarly to LTBI T_SCM cells in response to Mtb antigen.

(a) Schematic of T_SCM study where RSTR (n = 12) and LTBI (n = 12) PBMC from the household contact cohort were thawed, rested for 2 hours, stained with 5 µM CFSE, and stimulated with ESAT6/CFP10 or Mtb lysate overnight. (b) Gating strategy of activated naive-like CD69⁺CD154⁺CCR7⁺CD45RA⁺CD45RO⁻lymphocytes. (c) Representative flow cytometry showing sorted T_SCM cells from RSTR and LTBI expressing the proliferation marker CFSE after 7-day culture. Representive CD45RA and CCR7 staining of the CFSE^low population in response to ESAT6/CFP10 and Mtb lysate 7-day post-stimulation (d) Box plots showing the median and interquartile range of frequencies of T_SCM cells (CD95⁺CCR7⁺CD45RA⁺CD45RO⁻) at the time of sorting from PBMC stimulated with Mtb lysate or ESAT6/CFP10. Whiskers represent minima and maxima. (e) Frequencies of CFSE^lowCD95⁺ CD4⁺ T_SCM cells after 7-day culture. (f-g) Frequencies of proliferated cells in response to (f) Mtb lysate and (g) ESAT6/CFP10 stimulation. CM, central memory; EM, effector memory. Significance in d-g was calculated using the two-sided Wilcoxon rank-sum tests.