Extended Data Fig. 2: The composition of Mtb-specific T cell subsets.

(a) Gating strategy of ESAT6/CFP10-specific CD4⁺ T cells among PBMCs from the household contact cohort using index sort. T cells were identified as CD3⁺CD14⁻/CD19⁻. T cells positive for the TCRɑβ marker were gated on and separated into CD4⁺ or CD8⁺ subsets. Activated CD3⁺TCRαβ⁺ T cells were gated on using CD69, CD137, and CD154 markers. (b) Box plots showing the median and interquartile range of frequencies of ESAT6/CFP10-specific CD4⁺ T cell clusters defined in Fig. 2b among RSTR (n = 3) and LTBI (n = 4) donors in the household contact cohort using index sort and targeted transcriptomics. Whiskers represent minima and maxima. Statistical testing was not performed due to the small sample sizes. (c) Dimensionality reduction (t-SNE) projection of activated CD4⁺ T cell subsets sorted from PBMCs among the household contact cohort after stimulation with Mtb lysate for 12 hours.