Extended Data Fig. 5: Flow phenotyping of co-transferred exhausted EnhDel, Pdcd1-KO, and WT cells.
From: Epigenetic tuning of PD-1 expression improves exhausted T cell function and viral control
(a) Gating strategy for identification of live transferred EnhDel and Pdcd1-KO CD8+ T cells. (b) Representative flow cytometry plots of SLAMF6 versus TIM3 gating (left) and CX3CR1 gating (right) for Pdcd1-KO cells. (c) Representative flow cytometry plot of TNF staining in EnhDel (top left) versus Pdcd1-KO (bottom left) co-transfer, and EnhDel (top right) versus WT (bottom right) co-transfer. (d) Percentage of cells expressing TNF within the IFNγ+ population, quantified by subset. (Left) comparison of EnhDel versus Pdcd1-KO (n = 7); (right) EnhDel versus WT (n = 10). Two of two experiments combined, significance calculated using a paired Student’s t-test. Paired samples connected with lines. (e) Representative flow cytometry plot of KLRG1 staining in EnhDel (top left) versus Pdcd1-KO (bottom left) co-transfer, and EnhDel (top right) versus WT (bottom right) co-transfer. (f) Representative flow cytometry plot of Annexin V staining in EnhDel (top left) versus Pdcd1-KO (bottom left) co-transfer, and EnhDel (top right) versus WT (bottom right) co-transfer.
