Extended Data Fig. 7 | Nature Immunology

Extended Data Fig. 7

From: Efficacy of CTLA-4 checkpoint therapy is dependent on IL-21 signaling to mediate cytotoxic reprogramming of PD-1+CD8+ T cells

Extended Data Fig. 7

CD8+ T-cell responses during anti-PD-1 (nivolumab) or anti-CTLA-4 (ipilimumab) monotherapy in the CheckMate 238 cohort were analyzed by scRNASeq at week 1 and week 3 (n = 4 patients per treatment). (A) Pseudobulk PCA analysis indicates differential transcriptomic programing at week 3 between treatment conditions. PC1 and PC2 are shown, samples are colored by treatment regimen. (B) UMAP visualization of CD8+ T cells per analysis time point and treatment regimen. Clusters are indicated by color. (C) Expression of selected cytotoxic genes and IL-21 are depicted by violin plot stratified by treatment regimen. (D) Selected cytotoxic genes are visualized for cluster 3 cells by violin diagram. (E) Heatmap indicating differential gene expression is shown for clusters at week 3 time point.

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