Extended Data Fig. 3: Flow cytometry analysis of exhausted CD8+ T cell subsets.
From: The prostacyclin receptor PTGIR is a NRF2-dependent regulator of CD8+ T cell exhaustion
(a) Schematic of P14 cell adoptive transfer with LCMV infection. (b) Representative flow cytometry plots of donor CD8+/CD45.2+ WT and Keap1-/- P14 cells harvested from spleen of LCMV CL13-infected mice at 19 dpi. Bar graph quantifies the percent of CD8+/CD45.2+ WT and Keap1-/- P14 T cells in the spleen (mean±SEM, n = 4-5/sample). (c) Representative flow cytometry plots of progenitor exhausted 1 (Texprog1; LY108+/CD69+), progenitor exhausted 2 (Texprog2; LY108+/CD69-), intermediate (Texint; LY108-/CD69-) and terminally exhausted (Texterm; LY108-/CDD69+) donor CD8+/CD45.2+ WT and Keap1-/- P14 cells harvested from spleen of LCMV CL13-infected mice at 19 dpi. Bar graphs quantify the percent of respective subsets (mean±SEM, n = 4-5/sample). (d) Representative flow cytometry plots of PD-1+ versus TIM-3+ donor CD8+/CD45.2+ WT and Keap1-/- P14 cells harvested as in (a-c). Bar graphs quantify the percent of PD-1+/ TIM-3+ CD8+/CD45.2+ WT and Keap1-/- P14 T cells in the spleen (mean±SEM, n = 4-5/sample). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
