Extended Data Fig. 3: Heterogenous T_FH phenotypes display diverse cytokine profiles, similar to that of T_EFF and systemic cytokine milieu.

a–d Analysis of (a–c) draining lymph node cells and (d) serum from (a,b,d) wild-type and (c) IFNγ-GFPx4C13R reporter mice infected with indicated pathogens at early peak GC response. (a) Representative plots of CD4⁺CD44⁺CXCR5⁺PD-1⁺Ly6C⁻CD162⁻ T_FH cell IFNγ, IL-4, and IL-17 cytokine production. (b) Frequency of CD4⁺CD44⁺Ly6C⁺CD162⁺ T_EFF cell produced IFNg, IL-4, IL-17A. (c) IFNγ-GFPx4C13R reporter expression in CD4⁺CD44⁺CXCR5⁺PD-1⁺Ly6C⁻CD162⁻ T_FH cells following T. muris infection. Representative plots, frequency of cytokine reporter with T_FH population and displayed as parts of whole T_FH population. Inner slice displays cytokine coexpression. (d) Serum cytokine concentration (pg/mL) in serum (n = 10 mice per group). Cytokines displayed as parts of total cytokines analyzed. Data show experiments of 7–10 mice per group and mean ± SEM. Statistical tests: one-way ANOVA of multiple comparisons. **** P values are <0.0001 or otherwise indicated.

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