Extended Data Fig. 6: IL-21⁺ T_FH and IL-21⁻ T_FH cells are transcriptionally similar across diverse infections.

a, UpSet plot displaying intersects of genes expressed differentially between IL-21⁺ T_FH and IL-21⁻ T_FH cells from separate infections in bulk RNA-seq analysis of CD4⁺CD44⁺PD-1⁺CXCR5⁺FoxP3-RFP⁻IL-21–GFP⁺ T_FH and CD4⁺CD44⁺PD-1⁺CXCR5⁺ FoxP3-RFP⁻IL-21–GFP⁻ T_FH cells as in Fig. 3. Three genes intersecting across 4 infections indicated with arrow. b-g, Analysis of CD4⁺CD44⁺PD-1⁺CXCR5⁺CD162⁻ T_FH cells and CD4⁺CD44⁺CD162⁺ T_EFF cells from draining lymph nodes from wild-type mice infected with indicated pathogens, LCMV or influenza A at early peak GC response. (b) Gating strategy to identify T_EFF, IL-21⁻ T_FH, and IL-21⁺ T_FH cells. (c) Representative plots and histograms of CD62L, CD127, and Ly6C staining on IL-21⁻ T_FH, IL-21⁺ T_FH cells and T_EFF cells in LCMV infection. (d-g) Frequency and gMFI of CD62L⁺, CD127⁺, and Ly6C⁺ on (d,f) IL-21⁻ T_FH, IL-21⁺ T_FH cells, frequency of total T_FH population (e,g) T_FH and T_EFF, frequency of CD4⁺CD44⁺ population. (d-e) in LCMV infection (n = 8 mice per group), (f-g) in influenza A infection (n = 6 mice per group). Data show experiments of 4–6 mice per group and mean ± SEM. Statistical tests: Two-tailed unpaired Student’s t test. **** P values are <0.0001 or otherwise indicated.

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