Fig. 1: TNFΔARE/+ mice without B cells develop ileitis.
From: Distinct roles for B cell-derived LTα3 and LTα1β2 in TNF-mediated ileitis
WT, TNFΔARE/+ and μMT-TNFΔARE/+ BM chimeras were analyzed 16–17 weeks post-BMT. a, Schema describing BM chimera groups. b, Representative hematoxylin and eosin staining of the distal ileum of mice given WT, TNFΔARE/+ and B cell-deficient μMT-TNFΔARE/+ BM. Scale bars: 150 μm. c, Semiquantitative histological scoring on the distal ileum of BM chimera recipients (three independent experiments (WT (n = 9), TNFΔARE/+ and μMT-TNFΔARE/+ (n = 14)). d, Fold change in the amount of lipocalin-2 in the stool of mice normalized to the average amount per WT group per experiment (three independent experiments, WT (n = 14), TNFΔARE/+ and μMT-TNFΔARE/+ (n = 13)). e–k, Flow cytometry on single-cell suspensions of ileum to analyze the numbers and proportions of various immune cells including numbers of CD19+ cells (e), IgA+ plasma cells (f), neutrophils (g) and T cells (h), or the proportion of total CD4+ T cells (i), effector memory CD4+ T cells (j) or regulatory CD4+ T cells (k) (three independent experiments, WT (n = 15), TNFΔARE/+ and μMT-TNFΔARE/+ (n = 14)). Gating strategies for flow cytometry shown in Supplementary Figs. 1 and 3. All dataplots show mean ± s.e.m. Each symbol represents one mouse. One-way ANOVA with post hoc Tukey test (c), Kruskal–Wallis test with Dunn’s correction (d–k).
