Extended Data Fig. 3: Impact of deleting Lta or Ltb in mixed bone marrow chimeras or reagents targeting LTbR or LTa3 on lymphoid tissue organization.
From: Distinct roles for B cell-derived LTα3 and LTα1β2 in TNF-mediated ileitis
In Fig. 5, mixed BM chimeras were studied at 30–32 weeks after transplant, wherein WT recipient mice received μMT-TNFΔARE/+ BM at 90% and 10% marrow from WT, Lta−/− or Ltb−/− mice to resupply the B cell compartment with these respective genotypes. a: Vibratome sections of the spleen from these mice were made and immunostained for IgD, CD3, and CD21/CD35 to confirm that B cell deletions of Lta or Ltb altered splenic organization (Two independent experiments, n=2 spleens/group were imaged). b: Similar staining was made on spleen vibratome sections from TNFΔARE/+ mice that were treated with isotype control mAb, anti-LTα3 mAb, or LTβR-Ig for 2 days. Two independent experiments, n=2 spleens/group were imaged.
