Extended Data Fig. 4: Analysis of the role of LTα in nutrient absorption.
From: Distinct roles for B cell-derived LTα3 and LTα1β2 in TNF-mediated ileitis
a: Feces from a 24-h period of housing was collected from cages of mice that were recipients of WT/μMT-TNFΔARE/+ and Lta−/−/μMT-TNFΔARE/+ mixed bone marrow cells (cohort 1) or WT/μMT-TNFΔARE/+ and Ltb−/−/μMT-TNFΔARE/+ mixed bone marrow cells (cohort 2) to generate mice with ileitis with WT, LTα-deficient B cells (LTα−/− B cells), LTβ-deficient B cells (LTβ−/− B cells) with matched WT B cells compared in the respective cohorts. Feces from 4 to 5 mice in each cage were combined in each genotype of each cohort, dried and weighed, and subjected to bomb calorimetry. Data plot a combined value from each cage. b: TNFΔARE/+ mice were split into two cohorts and each was randomly assigned into groups (each 3 or 4 mice) that would receive anti-LTα3 mAb or isotype control. Fecal energy in the groups prior to administration of mAb versus the energy measured in feces over a 48-h treatment span (feces collected at 24 and 48 h in each cage) was assessed by bomb calorimetry. c,d: Plasma triglycerides were assessed after bolus gavage of olive oil in mice that were recipients of WT/μMT and Lta−/−/μMT mixed bone marrow cells (n=8 per group) (c) or in TNFΔARE/+ mice that were treated with anti-LTα3 or isotype control mAb for 2 days (n=4 per isotype, n=3 per anti-LTα3) (d). All mice in panels c and d were treated with poloxamer to inhibit lipolysis of triglycerides in the plasma during the assessment. Plasma measurements were performed as duplicates per mouse and timepoint and averaged together for a single value per mouse and timepoint. In c,d, data depicted show mean of the mouse groups ± SEM and each symbol represents a single time point per group.
