Extended Data Fig. 6: Assessment of the role of B cell-derived TNF in ileitis.
From: Distinct roles for B cell-derived LTα3 and LTα1β2 in TNF-mediated ileitis
Mixed BM chimeras wherein WT recipient mice received μMT-TNFΔARE/+ BM at 90% and 10% marrow from CD19Cre/+ x Tdtomatofl/fl mice (n = 3) or CD19Cre/+ x TNFfl/fl x Tdtomatofl/fl mice (n = 5) were studied at 32 weeks after BMT to assess the role of B cell-derived TNF on ileitis. a: Flow cytometry gating strategy for TdTomato+ CD19+cells gated otherwise as in Supplementary Figs. 1 and 2. Flow cytometry on single cell suspensions of the distal ileum of these mice compared the two genotypes in impacting neutrophil accumulation (b) or accumulation of T cells (c) which were not statistically significantly different based on a two-sided Mann-Whitney test. d: TLSs were formed in the mesentery of the mice. Stereoscope micrography reveals Tdtomato+ follicles near the vascular and lymphatic tracts. Bright field images were overlaid on fluorescence so that the blood flowing in vessels also appears red and the surrounding mesenteric adipose tissue reflects light. e: Assessment of TLS face area in the two genotypes with measurements made of 26 TLSs from mice bearing WT B cells and measurements from 8 TLSs made from mice with Tnf−/− (TNF KO) B cells. The size of TLSs were not statistically significantly different based on a two-sided Mann-Whitney test to compare them. In b,c, each symbol represents a single mouse and data represent mean ± SEM.
