Fig. 2: B cell-rich TLSs associate with ileitis-associated lymphatic transport blockade but not myeloid cell accumulation in the mesentery.
From: Distinct roles for B cell-derived LTα3 and LTα1β2 in TNF-mediated ileitis
WT, TNFΔARE/+ and μMT-TNFΔARE/+ BM chimeras 16–17 weeks post-BMT were studied. a–c, Representative whole-mount images (LYVE-1 red, CSF1R green, B220 blue) staining mice that received WT (a), TNFΔARE/+ (b) or μMT-TNFΔARE/+ (c) BM. Scale bars: 500 μm. d,e, The number of Ly6G+ neutrophils (d) and CD64+ MHCII+ macrophages (e) in the mesentery of TNFΔARE/+ and μMT-TNFΔARE/+ BM chimeric mice (three independent experiments, WT and TNFΔARE/+ (n = 14) and μMT-TNFΔARE/+ (n = 13)). f–h, Representative stereoscope images (left, brightfield and FITC channels; right, FITC channel alone) of the mesentery of anesthetized mice after FITC–dextran injection of 1–1.5 μl into the most distal Peyer’s patch. Mice given WT (f), TNFΔARE/+ (g) and μMT-TNFΔARE/+ (h) BM. Scale bars: 1.5 mm. i, Quantification of time to MLN in tracer experiments (three independent experiments, WT (n = 4), TNFΔARE/+ (n = 6) and μMT-TNFΔARE/+ (n = 4)). j–l, Number of TCRβ+ T cells (j), CD19+ B cells (k) and IgA+ plasma cells (l) in the mesentery of TNFΔARE/+ and μMT-TNFΔARE/+ BM chimeric mice (three independent experiments, WT and TNFΔARE/+ (n = 14) and μMT-TNFΔARE/+ (n = 13)). Gating strategy for flow cytometry shown in Supplementary Fig. 1. All dataplots show mean ± s.e.m. Each symbol represents one mouse; Kruskal–Wallis test with Dunn’s correction for d, e and i–l.
