Fig. 4: Secretory antibody partially protects against ileal inflammation in TNFΔARE/+ BM recipients, but this protection is limited only to the ileum.
From: Distinct roles for B cell-derived LTα3 and LTα1β2 in TNF-mediated ileitis
All panels show lethally irradiated Pigr−/− mice or Pigr+/+ mice given TNFΔARE/+ BMT for 16 weeks. a,b, Visualization of two projections (left, en face; right, cross-sectional) of a 3D rendering of ileum after whole-mount staining for IgA (red), smooth-muscle actin (white) and DAPI to identify nuclei (blue). a, Representative Pigr+/+ recipient mouse given TNFΔARE/+ BM. b, Representative Pigr−/− recipient mouse given TNFΔARE/+ BM; yellow arrow, abluminal accumulation of IgA in the Pigr−/− recipient. Scale bars: 100 μm. c, Micrograms of IgA per gram of stool in Pigr−/− versus Pigr+/+ recipients given TNFΔARE/+ BM. d, Fecal lipocalin-2 levels normalized to the average fecal lipocalin-2 amount in the Pigr+/+ TNFΔARE/+ BM controls per experiment. (two independent experiments, Pigr+/+ (n = 7), Pigr+/+ (n = 9). e,f, Flow cytometry on the ileum shows numbers of neutrophils (e) and T cells (f) (three independent experiments, Pigr+/+ (n = 8), Pigr−/− (n = 12)). g, Total body weight (four independent experiments, Pigr+/+ (n = 12), Pigr−/− (n = 15)). h, Number of monocytes in the blood (three independent experiments, Pigr+/+ (n = 8), Pigr−/− (n = 12)). i, Number of TLSs along the most distal branch of the mesentery (one experiment, Pigr+/+ (n = 3), Pigr−/− (n = 6)). Gating strategy for flow cytometry in Supplementary Figs. 1 and 2. All data in c–i show one mouse per symbol and depict mean ± s.e.m. Two-tailed Mann–Whitney test for all panels.
