Extended Data Fig. 1: Quantification of cell types in mouse blood or regions of the small intestine.
From: Distinct roles for B cell-derived LTα3 and LTα1β2 in TNF-mediated ileitis
a: Number of B cells in the blood by flow cytometry (four independent experiments, WT (n = 19), TNFΔARE/+ (n = 18), μMT-TNFΔARE/+ (n = 17)) b: Donor (CD45.2)+IgA+ plasma cells in the ileum by flow cytometry (three independent experiments, WT (n = 15), TNFΔARE/+ and μMT-TNFΔARE/+ (n = 14)). c: Percentages of FoxP3+RORγτ+ T regulatory cells. d: Number of neutrophils in the duodenum and jejunum by flow cytometry (2 independent experiments, WT and TNFΔARE/+ (n = 10) μMT-TNFΔARE/+ (n = 9)). e-g: Number of neutrophils (e) and monocytes (f) in the blood by flow cytometry (Four independent experiments, WT (n = 19), TNFΔARE/+ (n = 18) μMT-TNFΔARE/+ (n = 17)). g: Percentage of monocytes that are Ly6Chi in the blood (Three independent experiments, WT and TNFΔARE/+ (n = 14), μMT-TNFΔARE/+ (n = 12). Gating strategies for flow cytometry shown are in Supplementary Figs. 1–3. Kruskal-Wallis test on all with correction for multiple comparisons using Dunn’s test. All data depicted show mean ± SEM. Each symbol represents a single mouse.
