Fig. 3: Expression of low but functional levels of surface pre-TCR in human T-ALL.
From: Pre-TCR-targeted immunotherapy for T cell acute lymphoblastic leukemia
a,b, Representative pseudocolor plots of CD3 versus TCRαβ expression displayed by T-ALL3, T-ALL17, T-ALL18 (a) and T-ALL2 (b) human samples recovered from spleen xenografts of NSG mice from two independent experiments (left histograms). Representative monoparametric histograms on the right show surface pre-TCR expression levels on electronically gated CD3loTCRαβneg, TCRαβ+ and CD3neg T-ALL cells analyzed with anti-pTα monoclonal antibody with either goat anti-mouse FITC (a) or biotin-coupled anti-mouse (Fab′)2 plus PE-coupled streptavidin (b). Numbers indicate percentages of positive cells. Gray shaded histograms show background staining with an irrelevant isotype-matched antibody. c, Immunoblot analysis of ERK and S6 phosphorylation in pre-TCR+ (T-ALL3 and T-ALL17) or TCRαβ+ (T-ALL2) T-ALLs, activated either with anti-pTα or with anti-CD3ε monoclonal antibodies for the indicated times. One experiment per T-ALL sample was performed. Total ERK and S6 expression is shown as the loading control.
