Extended Data Fig. 1: Expression of pre-TCR in human T-ALLs.
From: Pre-TCR-targeted immunotherapy for T cell acute lymphoblastic leukemia
(A) qPCR analysis of PTCRA mRNA expression normalized to GAPDH expression of T-ALL patient samples recovered from spleen xenografts of NSG mice (n = 22, Table 1). Data are shown as mean of technical triplicates relative to values derived from healthy PB T cell samples (n = 2). Dashed line shows relative PTCRA expression in the pre-TCR+ SupT1 cell line included as positive control. (B-E) Normalized PTCRA expression values derived from public RNA-seq raw data of a cohort of >1300 T-ALL patient samples15 and >100 B, T and myeloid PB cells from healthy donors44. Data are shown as normalized counts +1 either from healthy B, T, and myeloid peripheral blood cells, and total T-ALL samples (B) or from T-ALL samples (C-E) defined by ETP status (C, ****P < 0.0001), genetic subtypes grouped by their similarity to the expression signature of control haematopoietic cells (D, ****P < 0.0001), and TRA, TRB, TRG and TRD gene rearrangements (E, ****P < 0.0001). Dashed line shows cut-off value for PTCRA expression. Median and quartiles values within violin plots are indicated by solid and dashed lines, respectively. Data were analysed by one-way ANOVA with Dunn’s multiple comparison test.
