Extended Data Fig. 5: Spatial immune landscape of pediatric LGG, as Related to Fig. 4.
From: A spatial map of MAPK-activated immunosuppressive myeloid populations in pediatric low-grade glioma
a. Heatmap of percentages of cell types represented across 10 CNs discovered in pLGG cores (n = 120). b. Percentage of CNs comparing the different tumor types (n = 120), locations (n = 117), patient age (n = 120) and sex (n = 118), and genomic alterations (n = 115). CN1 was significantly different between PA and GG, between BRAF-fusion and both other mutation statuses, and between all locations. c. The percentage of all immune cells in the TME was plotted and distributed based on tumor location (n = 117). Data are presented as mean, with all samples plotted. d. Total brain or tumor and immune, presented as a proportion of all cells in the TME, distributed by tumor location in the brain. Data are presented as the mean, with all samples plotted, statistical analysis conducted using the two-way ANOVA. Cerebellum n = 72, Cortex n = 18, Midline n = 16, Optic pathway n = 11. ****p < 0.001, **p < 0.01, *p < 0.05. e. Samples were sorted as described in Fig. 4i and depicted individually by location. The upper and lower quartiles are depicted by a red or blue box, respectively. f. Heat map indicates IMC spatial analysis among cell phenotypes and their patterns of cell-cell interactions or avoidance (blue) in pLGG tumors, determined by pairwise scores. The determination is based on pairwise scores, and only interactions/avoidances exceeding 50% (random chance) are displayed. Interpretation should be done row-to-column, where rows indicate the relationship of a specific cell type, and columns represent the relationship of that cell type with other surrounding cell types. g. Cell-cell interaction network depicting the interaction degree of a given cell population with all other cell populations in in the cerebellum, cortex, or midline vs optic pathway glioma (OPG). The dot size represents the proportion of each cell population in the entire cohort, while the edge thickness represents the degree of interaction/avoidance (two-sided test).
