Extended Data Fig. 1: Imaging Mass Cytometry to profile the tumor microenvironment of pLGG, as Related to Fig. 1.
From: A spatial map of MAPK-activated immunosuppressive myeloid populations in pediatric low-grade glioma
a. Illustration of the data acquisition workflow and overview of the data analysis pipeline used to characterize human pediatric low-grade glioma tumors (PA = 113 and GG = 7) by IMC. b. Antibodies used to classify the different cell types in the tumor microenvironment cell lineage assignment tree. c. Heat map showing relative average expression of markers across cell populations identified by IMC. d. Immunofluorescence validation of target detection of antibodies that underwent metal-conjugated in-house and lacked pre-validation from literature. Human tonsil (CD206) or brain tumor tissues (GFAP, SOX2, MAP2, SOX10 and Olig2) were used. e. Representative IMC images of human tonsil tissue used as a control. Macrophages (CD68+), monocytes (CD14 + CD16+), B cells (CD20+), T cells (CD45 + CD3 + CD4 + CD8+), dendritic cells (HLA-DR+CD11c+), and endothelial cells (CD31+) are represented. Antibodies that did not show consistent staining by IMC were excluded from the analysis.
