Extended Data Fig. 3: A myeloid immunophenotype signature is significantly associated with PFS in pLGG, as Related to Fig. 2.
From: A spatial map of MAPK-activated immunosuppressive myeloid populations in pediatric low-grade glioma
a. Cell populations and their association with progression-free survival (uncorrected log-rank test) in the IMC cohort (n = 92 samples). High = percent of cell population higher than the median, Low = percent of cell population lower than the median. b. Kaplan-Meier curve of IMC samples (n = 92) grouped based on the patients’ Cl/Atl ratio (uncorrected log-rank test). c. Kaplan-Meier curve of IMC samples (n = 85) grouped based on the patients’ resection status. Is also indicated the adjusted pvalue (BH test) for all pairwise comparisons using the Log-Rank test. d. Forest plot depicting the hazard ratio (HR) calculated in a univariate Cox proportional hazards model evaluating which clinico-molecular features are significantly associated with PFS in our pLGG cohort (n = 120). Is also depicted the upper and lower 95% confidence intervals for the hazard ratio. e-f. Kaplan-Meier curve of IMC samples from patients at risk of progression, either without gross-total resection (GTR) (E – n = 70 samples), or based on location and resection (F – n = 31) and grouped based on their immunophenotype defined by the cell populations significantly associated with PFS. Is also indicated the adjusted pvalue (BH test) for all pairwise comparisons using Log-Rank test. g. Kaplan-Meier curve of IMC samples (n = 92) grouped based on their immunophenotype defined by the cell populations significantly associated with PFS (5 groups). h. Heatmap summarizing the adjusted p-values (BH correction) of all pairwise comparison using the log-rank test. i. Connected dot plot depicting all combinations of one, two, three, or four cell populations to calculate the immunophenotype signature and their associated PFS log-rank test p-value in the survival analysis. j. Table summarizing the p-value (log-rank test) and adj-p-val (Bonferonni correction) from the Kaplan-Meier analysis looking at the association of the genes associated with the immune phenotype and PFS in our cohort.
