Extended Data Fig. 2: Initial analysis of the single-cell chromatin of splenic pDCs.
From: Tonic type I interferon signaling optimizes the antiviral function of plasmacytoid dendritic cells
Sorted pDCs from pooled spleens of naive mice were subjected to multiome analysis by single-nuclei RNA-seq and ATAC-seq (Fig. 1), and the latter was analyzed. (a) UMAP plot of cells based on ATAC-Seq reads, indicating the original 9 clusters. (b) Correlation heatmap of the original 9 clusters and after cluster reduction, with the corresponding Leiden clustering resolutions indicated. The heatmap on the right corresponds to the four Ma clusters in Fig. 1e. (c) The projection of Ma clusters onto the UMAP plot based on RNA-Seq from Fig. 1a. Shown are combined projections of all clusters (left panel) and feature plots of individual Mt clusters (right panel). (d) The fraction of Ma clusters within the total dataset and within each Mt cluster.
